Kirchner, Thomas W.; Niehaus, Markus; Debener, Thomas; Schenk, Marco K.; Herde, Marco
(San Francisco, CA : Public Library of Science, 2017)
A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the ...